For immunoblot evaluation of IL-6 and IL-1b, 4-weeks-previous male mice were being applied for a one injection and have been sacrificed 6 several hours later as described over
Brains from 6, P1-three neonatal mice were gathered in L-15 Leibovitz medium (Biological industries, Israel). Following elimination of the meninges, the cortexes had been dissected, washed with cold HBSS (Biological…