The supernatant was blended with 70 ml of 17% (W/ V) polyethylene glycol 6000 (PEG), and .157 M NaCl that contains 2% casamino acids to precipitate the chromosomal DNA for the selection of DNA-related proteins
Electrophoresis mobility shift assay demonstrating DNA binding. Electrophoresis mobility shift assay utilizing the purified CBS domain protein (CBSDP) and a 309 bp DNA fragment from upstream of the pepV gene…