Figure legends. For some experiments the information was plotted non-categorically in line graphs of the accumulated % response around the Y-axis versus Tubacin supplier latency on the X-axis, and tested…
Phs of accumulated percent response as a function of measured latency. DOI: 10.7554/eLife.10735.017 Figure supplement two. Genetic epistasis tests among DTKR and TNF pathway. DOI: 10.7554/eLife.10735.018 Figure supplement three. Schematic…
In dPob4 photoreceptor cells, indicating that dPob is essential for the early stage of Rh1 biosynthesis prior to chromophore binding within the ER. NinaA, the rhodopsin-specific peptidyl-prolyl-cis-trans-isomerase, is often a…
Confocal and Photoshop software program. Immunolabeled retinas were commonly examined with a vertical resolution of 0.four.two m below normal line-scan and frame-scan modes and additional examined with confocal Airyscan protocol…
Too as other chemicals, were purchased from Sigma-Aldrich unless stated otherwise. A temperature control unit (TC 324B, Warner Instruments, CT) was utilized to control and monitor the temperature on the…
Centrifugation for 20 min at 10,500 rpm (13,000 ) within the SS34 rotor of a refrigerated centrifuge (Sorvall RC-5B). Protein concentration in the clarified lysate was measured utilizing BCA reagent…
Aposed with TKexpressing cells inside the VNC. Arrows, regions where GFP-expressing axons are closely aligned with DTK-expressing axons. DOI: ten.7554/eLife.10735.009 The following figure supplement is accessible for figure 2: Figure…
Get rid of the URA plasmid carrying the wild-type, full-length copy of Tim44, no viable cells were obtained (Figure 1B). A plasmid carrying the full-length copy of Tim44 enabled growth…
Gure 6A). To appear for interaction partners of the core domains, each domains now lacked the segment containing A1 and A2 helices. Purified proteins have been covalently coupled for the…
Ed from FL and N+C cells were analyzed by SDS AGE, followed by immunoblotting against depicted Vitamin K2 Protocol mitochondrial proteins. DOI: 10.7554/eLife.11897.Banerjee et al. eLife 2015;four:e11897. DOI: ten.7554/eLife.five ofResearch…