As outlined by manufacturer's directions (Qiagen). RNA good quality was determined by Agilent 2100 Bioanalyzer employing the Pico Chip (Agilent, Santa Clara, CA, USA). Samples with RIN 7 had been…
Phs of accumulated percent response as a function of measured latency. DOI: 10.7554/eLife.10735.017 Figure supplement two. Genetic epistasis tests among DTKR and TNF pathway. DOI: ten.7554/eLife.10735.018 Figure supplement three. Schematic…
S Hog1 binding to and regulation of Fps1, and Rgc27A cannot be displaced from Fps1 since it can not be phosphorylated by Hog1; both mutations render the channel constitutively open…
Ng a certain antibody (Niles et al., 2012) that monitors phosphorylation of Ypk1 in the exact same web site (Figure 1--figure supplement 4A). Making use of Ypk17A, which also permits…
An osmolyte to counterbalance the external higher osmolarity. (B) Unstressed situation (leading), active TORC2-Ypk1 keeps intracellular glycerol level low by inhibition of Gpd1 (Lee et al., 2012) and Figure 4.…
Moting processes that stimulate retrieval of excess plasma membrane (Guiney et al., 2015). Despite the fact that both CN-deficient and hog1 cells are really sensitive for the ionic imbalances caused…
Phs of accumulated % response as a function of measured latency. DOI: ten.7554/eLife.10735.017 Figure supplement 2. Genetic epistasis tests between DTKR and TNF pathway. DOI: 10.7554/eLife.10735.018 Figure supplement three. Schematic…
Sed against the human glutamine synthetase aa 173 and recognized a band at 45 kDa, constant together with the predicted molecular weight of GS. The specificity of these main antibodies…
An osmolyte to counterbalance the external higher osmolarity. (B) Unstressed condition (prime), active TORC2-Ypk1 keeps intracellular glycerol level low by inhibition of Gpd1 (Lee et al., 2012) and Figure 4.…
In A-deficient medium within a Rh1-driven experiment. For heat-shock driven expression, newly eclosed adult fly flies had been incubated at 37 for 45 min each day just before preparation. Within…