Ith concentrate on the evaluation of their influence on CLL Fc Receptor Like 2 (FCRL2) Proteins manufacturer immune 4-1BB/CD137 Proteins supplier escape. Altogether, this study will give insight in to the precise immune and stromal cells involved in CLL improvement, with emphasis on their involvement in tumour-derived tiny Ev-mediated tumour immune escape. Funding: This project is funded by the Fonds National de la Recherche (FNR) INTER/DFG/16/11509946/EVRNA/Moussay. Sandrine Pierson and J e Paggetti are supported by the FNR INTER/DFG/16/11509946/EV-RNA/ Moussay. Ernesto Gargiulo is supported by the grant FNR Luxembourg PRIDE15/10675146/CANBIO.PT06.Interaction by way of exosome miRNAs in between myelodysplatic cell and regular Treg Tatsuki Shibuta, Yukichi Takada and Tsukuru Umemura International University of Well being and Welfare, Okawa City, Japanregulatory T cells (Treg) that were sorted from standard peripheral blood. The exosomes were detected in cytosol of Treg by fluorescent microscopy. Microarray evaluation of miRNAs in Treg intaking MDS-exosomes showed that significant increases of 9 miRNAs in MDS-exosomes. The conditioned medium of MDSexosomes treated Treg culture lowered the population of activated CD4 cells (CD38 positive cells was 39 ; control 68). Summary/Conclusion: Our data suggested that exosomes from MDS cells impacted the function of regulatory T cells via miRNA transfer. MDS exosomes may well effect on immune cells to avoid the exclusion from cancer-immune system, and may possibly be a target for the new therapies or diagnostic strategies. Funding: This work was supported in element by a grant in the Japan Society for the Promotion of Science (JSPS KAKENHI Grant Number: JP17K09020 and 17H07059).PT06.Mechanism of antitumor immunity activation by `artificial neoantigen’-presenting exosomes Yoshiyuki Koyamaa, Tomoko Itoa, Masazumi Eriguchia, Aya Hasegawab, Wakana Ouchic, Toshio Inabab and Kikuya SugiurabaIntroduction: Myelodysplastic Syndrome (MDS) is actually a clonalhematopoietic disease and develops leukaemia in some situations. Therefore, MDS is often a malignant hematopoietic disease and its prevalence ratio is growing in Japan. Hematopoietic microenvironment which include bone marrow niche is really a crucial factor for preserving leukaemic stem cells. To understand mechanisms of interactions in between leukaemic stem cells and microenvironment is important for the therapy of hematopoietic malignancies. In this study, to create the new therapies and diagnostic strategies for MDS, we focused around the impact of exosomes released from MDS cells on peripheral T lymphocytes. Solutions: MDS cell line (MDS-L) was kindly provided by Kasawaki Medical University and regular peripheral blood mononuclear cells have been obtained from healthier volunteer donors. Exosomes from MDS cells were purified by using miRCURY Exosome Cell/Urine/CSF Kit and labelled by PKH67. Extracted miRNAs were analysed by microarray system (Genopal, Mitsubishi Chemical, Japan). Cell surface antigens were analysed by FACS Aria II and fluorescence conjugated antibodies. Outcomes: miRNA-microarray evaluation showed that nine miRNAs were abundant in exosomes from MDS cells and were not detected in MDS cells. Exosomes labelled with PKH67 dye have been added to liquid culture ofJapan Anti-tuberculosis Association, Shin-Yamanote Hospital, Tokyo, Japan; Osaka Prefecture University, Osaka, Japan; cOsaka Prefecture University, Tokyo, JapanbIntroduction: Tumour-derived exosomes are recognized to possess very same antigens because the parent tumour cells, and had been expected as cancer vaccines. Nevertheless, therapy with these exosomes frequently failed to elicit.