S gene expression was considerably enhanced compared to PRGF alone. One-way ANOVA, Tukey’s a number of comparisons test, expression was drastically elevated compared to PRGF alone. One-way ANOVA, Tukey’s IFN-lambda Proteins Biological Activity various comparisons test, p 0.05 (n = four). (B) p62/sqstm1 protein expression measured by Western blotting. Final results indicate that PRGF plus blue p 0.05 (n = four). (B) p62/sqstm1 protein expression measured by Western blotting. Final results indicate that PRGF plus blue light led to a important boost within the expression of this marker in comparison with the control and PRGF remedies. One-way light led to a substantial enhance inside the expression of this marker when compared with the manage and PRGF treatments. One-way ANOVA, Tukey’s several comparisons test, p 0.05, p 0.005 (n = 4). ANOVA, Tukey’s various comparisons test, p 0.05, p 0.005 (n = four).Biomolecules 2021, 11,three.3. Atg5 three.three. Atg5 The subsequent marker of autophagy examined, Atg5, showed enhanced expression in the The following marker of autophagy examined, Atg5, showed enhanced expression inside the presence of PRGF (Figure five). This protein acts in thethe initial stages of autophagosome presence of PRGF (Figure 5). This protein acts in initial stages of autophagosome forformation, suggesting expression might be simpler to study in the initial stages of Matrix Metalloproteinases Proteins Purity & Documentation exposure mation, suggesting its its expression might be less difficult to study in theinitial stages of exposure to blue light and/or PRGF remedy. Our Western blots, nonetheless, revealed the improved to blue light and/or PRGF treatment. Our Western blots, even so, revealed the enhanced production of this marker in response to blue light, each alone and in combination with production of this marker in response to blue light, each alone and in combination with PRGF. This could recommend that the presence of this marker is induced in the initial stages 8 of 17 PRGF. This could suggest that the presence of this marker is induced in the initial stages of autophagy by means of its gene expression but that the protein remains activated till the autophagy via its gene expression but that the protein remains activated until the of final stages. final stages.Figure 5. Atg5 gene expression, and protein expression relative for the expression of actin. (A) Atg5 gene expression Figure five. by qPCR. expression, and protein expression of PRGF, its gene expression was (A) Atg5 gene expression measmeasuredAtg5 gene Results indicate that in the presence relative for the expression of actin.significantly elevated compared ured blue light therapy, combined the presence of PRGF, its gene expression was considerably enhanced compared to to theby qPCR. Results indicate that inor not with PRGF. One-way ANOVA, Tukey’s multiple comparisons test, p 0.05 the 4). (B) Atg5 protein combined or not with PRGF. One-way ANOVA, indicate a number of light, alone or combined with (n =blue light remedy,expression measured by Western blotting. Benefits Tukey’s that blue comparisons test, p 0.05 (n = 4). (B) Atg5 protein expression measured by Western blotting. Final results indicate that blue light, alone or combined with PRGF, led to a considerable raise in the expression of this marker compared to the PRGF remedy. One-way ANOVA, PRGF, led to a substantial enhance within the expression of this marker in comparison to the PRGF remedy. One-way ANOVA, Tukey’s various comparisons test, p 0.005 (n = 4). Tukey’s various comparisons test, p 0.005 (n = four).3.4. LC3 The gene expression of LC3 was located si.