He impact of CM supplementation. To make the study much more clinically relevant, mature adipocytes must be used to show how these mature cells will react to hypoxia and CM supplementation. In addition, long-term research under hypoxia utilizing 3D printed scaffolds with each other with a bioreactor technique would also deliver an interesting point of view.any other stressful environment tends to induce a anxiety response towards the cells.37 Within this case, HPADs seemed to react towards the pressure of hypoxia by differentiating and advertising angiogenesis. Despite the fact that CM supplementation alone also leads HPADs to react similarly, CM/HYP increases the viability and fold transform of key gene markers drastically. We believe the obtaining is very important given the hypoxia clinicallyCONC LU SIONSBased around the benefits of this study, it could be concluded that Gtn-FA hydrogel crosslinked with laccase correctly produces a hypoxic atmosphere as validated by EPROI. Following exposure to a hypoxic environment, amniotic membrane supplementation drastically increasedMAGANA ET AL.viability and key gene markers for adipocyte differentiation and functionality of cultured preadipocytes. ACKNOWLEDGMENTS The authors acknowledge the monetary assistance from the Blazer Foundation, the OSF St Anthony Hospital Foundation, Office of Study Bridge funding (Bijukumar) and the Healthcare Biotechnology Adiponectin Proteins medchemexpress System of Department of Biomedical Sciences, Rockford. O2M Technologies acknowledges the help of SBIR grants from NSF 1819583, 2028829, and NIH LAIR-1/CD305 Proteins Storage & Stability R43CA224840, R44CA224840. Boris Epel discloses financial interests in O2M Technologies. The authors considerably appreciated the support from Smith and Nephew by giving sufficient cryopreserved placental membrane for this study. Because of Ritu Padaria, Masters in Healthcare Biotechnology for her support in figure arrangement. Authors also acknowledge Dr. Robin Pourzal, Rush University Medical Center for supporting FTIR analysis in this study. Information AVAI LAB ILITY S TATEMENT The information that support the findings of this study are accessible from the corresponding author upon reasonable request. ORCID Divya Bijukumar RE FE R ENC E S1. Jeong JH. Current advancements in autologous fat grafting. Arch Aesthetic Plast Surg. 2014;20(1):3-7. two. Abboud MH, Dibo SA, Abboud NM. Power-assisted liposuction and Lipofilling: strategies and expertise in large-volume fat grafting. Aesthet Surg J. 2020;40:180-190. 3. Khouri RKJ, Khouri RK. Current clinical applications of fat grafting. Plast Reconstr Surg. 2017;140(three):466e-486e. four. Gutowski KA, ASPS Fat Graft Activity Force. Current applications and safety of autologous fat grafts: a report of the ASPS fat graft task force. Plast Reconstr Surg. 2009;124(1):272-280. 5. Bank J, Fuller S, Henry G, Zachary L. Fat grafting for the hand in sufferers with Raynaud phenomenon: a novel therapeutic modality. Plast Reconstr Surg. 2014;133(5):1109-1118. 6. Pers Y-M, Rackwitz L, Ferreira R, et al. Adipose mesenchymal stromal cell-based therapy for extreme osteoarthritis of your knee: a phase I dose-escalation trial. Stem Cells Transl Med. 2016;5(7):847-856. 7. Haahr MK, Jensen CH, Toyserkani NM, et al. Safety and potential impact of a single Intracavernous injection of autologous adiposederived regenerative cells in individuals with erectile dysfunction following radical prostatectomy: An open-label phase I clinical trial. EBioMedicine. 2016;five:204-210. eight. CondGreen A, Marano AA, Lee ES, et al. Fat grafting and adiposederived regenerative cells in burn wound heali.