E validated by confirming corresponding marker proteins (CD9; EVs, apoA-I; HDL, apoB; LDL/ VLDL). As a result of lipidomic evaluation, we identified 264 MMP-13 MedChemExpress lipids in plasma EVs, HDL and LDL/VLDL fractions. We also identified that EVs showed strikingly higher levels of lyso-glycerophospholipids than HDL and LDL/VLDL. Furthermore, compared with EVs, larger sphiongolipid species levels have been observed in LDL/ VLDL, when polyunsaturated phosphatidylcholine were highly detected in HDL. Similar profiles were also observed in every fraction derived from human serum. Summary/conclusion: Lipidomic profiling demonstrates that EVs has a unique lipid profile compared with lipoprotein particles, even though the biological which means of these differences needs to be further evaluated in future studies. Nevertheless, the strategy presented in this study is often beneficial for lipid biomarker screening for EVs also as lipoprotein particles derived from both plasma and serum for human illnesses. Funding: Japan Agency for Medical Research and DevelopmentLBT01.Enhancing extracellular vesicle isolation of human plasma verified by high resolution lipidomics Amani M. Batarseha, Alex Chenb, Kim Ekroosc, Susannah Hallald, Kimberley Kaufmane and Michael Marianif BCAL Dx, Eveleigh, NSW, Australia 2015, Eveleigh, Australia; bThermo Fisher Scientific, Scoresby, VIC, Australia 3179, Scoresby, Australia; c Lipidomics Consulting Ltd., Esbo, Finland 02230, Esbo, Finland; d Discipline of Pathology, Brain and Thoughts Centre, Adenosine A2A receptor (A2AR) Antagonist custom synthesis Sydney Medical School, University of Sydney, Camperdown, NSW, Australia 2050, Camperdown, Australia; e1-Department of Neurosurgery, Chris O’Brien Lifehouse, Camperdown, NSW, Australia 2050, 2-Discipline of Pathology, Brain and Mind Centre, Sydney Medical College, University of Sydney, Camperdown, NSW, Australia 2050, Camperdown, Australia; fThermo Fisher Scientific, North Ryde, NSW, Australia 2113, North Ryde, AustraliaaIntroduction: Extracellular vesicles (EVs) are lipid bilayer nano-vesicles current in many biofluids, and regarded as worthwhile sources for biomarker. To information, the primary target field of preceding biomarker studies on EVs are proteome and transcriptome. Meanwhile, liquid chromatography coupled with higher resolution mass spectrometry (LC-MS) has recently been employed to study comprehensive lipid profiles of in vitro EVs and their parental cells. However, lipid profile of EVs in biolfluids, specifically blood specimens including plasma and serum, has not been well-characterized. To work with manage information for EVs, we aimed to characterize lipid profile of EVs in human healthier plasma and serum, and to examine their lipid profile with that of other lipid-containing particles in blood,Introduction: Extracellular vesicles (EVs) are secreted from many cell kinds and play crucial roles in intercellular communication. EVs carry a variety of biomolecules that reflect the identity and molecular stateISEV2019 ABSTRACT BOOKaof their parental cell and are located in biological fluids. Omics research have extensively focused on characterisation in the protein and nucleic acid cargo of EVs while lipids are much less studied. EVs are increasingly getting utilised in illness diagnosis as they may be considered to carry beneficial information regarding the illness state. Hence, novel illness biomarkers may be identified EV lipidomes. Techniques: EVs were enriched from 1ml regular human plasma samples applying ultracentrifugation (UC), deemed the gold standard method for EV enrichment, and size exclusion chrom.