Ng default parameters and 1000 bootstraps with RAxML v8.2.12 [49]. The 16s rRNA
Ng default parameters and 1000 bootstraps with RAxML v8.two.12 [49]. The 16s rRNA gene of Staphylococcus aureus (RefSeq ID: GCF_000013425.1) was utilized as an outgroup. The origin of replication (OriC) was identified employing DoriC database [50] and Mauve aligner [51]. Pairwise genomic comparison of strain BSE6.1 was produced with three other associated genomes. Dotplots had been constructed with minimap2 primarily based pairwise alignment using D-Genies [52]. Prokka v1.14.6 was utilised to carry out a regional de novo annotation [53]. Pan-genome comparison with 100 related genomes ( 90 16S nucleotide identity; 80 whole-genome aligned fraction identity) was created working with the pan-genome tool at KBase server [46]. Gene clusters associated towards the secondary metabolite biosynthesis have been identified using the antiSMASH five.0 DYRK Purity & Documentation pipeline [54]. The red pigmentproducing gene cluster of BSE6.1 was compared with that of S. coelicolor A3(2), Serratia, and Hahella utilizing the multigene BLAST tool [55]. The distribution of numerous coding sequences (CDS) and gene clusters across the genome was plotted utilizing Circos [56].Microorganisms 2021, 9, x FOR PEER REVIEW4 ofMicroorganisms 2021, 9,A3(2), Serratia, and Hahella using the multigene BLAST tool [55]. The distribution17 vari4 of of ous coding sequences (CDS) and gene clusters across the genome was plotted utilizing Circos [56].Figure 1. Workflow and pipeline of toolsand pipeline of tools employed reads into a genome reads into a genome and additional Figure 1. Workflow utilized to Aurora C Synonyms assemble the raw to assemble the raw and further evaluation from the assembled genome. analysis from the assembled genome.three. Results and Discussion Strain BSE6.1 developed a pink-colored development in Minimal broth with 2 NaCl and red pigmentation in all other compatible media. Pale pink to reddish colonies with whiteMicroorganisms 2021, 9, x FOR PEER REVIEW5 of3. Results and DiscussionMicroorganisms 2021, 9,Strain BSE6.1 made a pink-colored growth in Minimal broth with 2 NaCl and red pigmentation in all other compatible media. Pale pink to reddish colonies with white powdery spores had been observed immediately after 7 or 10 days of incubation. Salt tolerance was observed up to a rangeobserved after 7 orbacterium incubation. Salt tolerance was observed powdery spores were of two to 7 . This ten days of was positive for catalase and oxidase activities. In our earlier study, strain BSE6.1 showed possible antibacterial activity against as much as a selection of 2 to 7 . This bacterium was good for catalase and oxidase activities. diverse human pathogens and also displayed a powerful ability toactivity against diverse In our earlier study, strain BSE6.1 showed possible antibacterial stain epidermis and parenchyma cells of Tridax procumbens stem [25]. The maximum pigmentand parenchyma human pathogens as well as displayed a robust capability to stain epidermis production was observed at 29procumbens stem [25]. The maximum pigmentfor its development was 38 (Figcells of Tridax , plus the maximum temperature tolerance production was observed at ure2). and the maximum temperature in the red for its growth was 38 Cobserved2). The 29 C, The peak absorption spectrum tolerance pigment of BSE6.1 was (Figure at 528 nm [25]. peak absorption spectrum of your red pigment of BSE6.1 was observed at 528 nm [25].five ofFigure Morphological and biochemical Figure 2. Morphological and biochemical traits of Streptomyces sp. strain BSE6.1.Identification from the red pigment by means of thin layer chromatography (TLC), FourierIdentification of the red.