Product Name :
C-His tag Nanoselector Agarose
Applications:
IP,CHIP,MS,Purification
Reactivity :
His tag
Conjugate:
Agarose
Advantages :
· Consistent and reproducible results· No heavy & light antibody chains · Extraordinary binding, even under harsh conditions· High affinity · Short Incubation (5-30 min)
Description:
| Description: | C-His tag Nanoselector Agarose have been specifically designed to bind His tag-fusion proteins. His tag Nanoselector Agarose is based on small high-affinity recombinant alpaca antibody fragments covalently coupled to the surface of Agarose. His tag Nanoselector Agarose is an ideal tool to isolate or purify His tag-fusion proteins fast and efficiently. | | Ligand: Anti-His tag(C-terminal) single domain antibody fragment (VHH, Nanobody) | Bead size: ~ 40 µm | Reactivity: The C-His tag-Nanoselector recognizes the His-tag sequence motif HHHHHH at the C-terminus of the fusion protein. | Binding capacity: High binding capacity, 10 µL slurry bind about 20µg of recombinant GFP-His protein. | Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze. | Storage buffer: Formulation 50 % slurry in PBS containing 20 % Ethanol. | Background:His-tag, or polyhistidine tag, comprises a consecutive series of six to ten histidine residues. The size of the His tag is small and it hardly affects the functionality of the fusion protein.{{910463-68-2} MedChemExpress|{910463-68-2} Technical Information|{910463-68-2} In Vitro|{910463-68-2} manufacturer} His tag is widely used for many recombinant proteins to facilitate purification, and allows researchers to purify the protein of interest from cell lysate or mixture of many proteins easily . His tag Nanoselector Agarose has been specifically designed to bind His tag. Due to the single-chain nature of sdAbs and their stable and covalent attachment, no leakage of light and heavy chains is observed during elution with SDS sample buffer.{{2253733-10-5} MedChemExpress|{2253733-10-5} Protocol|{2253733-10-5} In Vitro|{2253733-10-5} custom synthesis} His tag Nanoselector Agarose thus features high affinity and superior capacity for His tag fusion proteins while showing negligible non-specific background.PMID:29083726 His tag Nanoselector Agarose is compatible not only with physiological buffers but also with high stringency buffers which provides great freedom to adjust the binding and washing conditions to the experimental needs.
Description2 :
Immunogen:
Host :
Isotype:
Purity :
Buffer :
Storage :
Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.
Function:
Immunoprecipitation (IP)/Co-IP Mass spectrometry (MS) Enzyme activity measurements