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[pThr286]Ca2+/calmodulin-dependent protein kinase II (rat) (α subunit) monoclonal antibody (22B1)
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| Alternative Name CaM kinase II α subunit, CAMK2 α subunit | Application ELISA, Flow Cytometry, ICC, IF, IP, WB | Clone 22B1 | Formulation Liquid. In PBS containing 0.05% sodium azide. | Host Mouse | Immunogen Synthetic peptide corresponding to aa 281-294 (M281HRQET(PSO3)VDCLKKFN294) of the α-subunit of mouse and rat CaM kinase II (Ca2+/Calmodulin-dependent Protein Kinase II) thiophosphorylated at (Thr286). | Isotype IgG1 | Recommendation Dilutions/Conditions Flow Cytometry (FACS, 1μg 106 cells)Immunocytochemistry (1:100)Immunofluorescence (1:100)Western Blot (1:2,000)Suggested dilutions/conditions may not be available for all applications.{{722543-31-9} web|{722543-31-9} Purity & Documentation|{722543-31-9} In Vivo|{722543-31-9} custom synthesis} Optimal conditions must be determined individually for each application.{{1416775-46-6} web|{1416775-46-6} Purity & Documentation|{1416775-46-6} Description|{1416775-46-6} supplier} | Species Reactivity Human, Mouse, Rat | Specificity Recognizes the phosphorylated α-subunit of CaM kinase II.PMID:31194459 This antibody does not detect the non-phosphorylated α-subunit of rat CaM kinase II nor the ~60kDa β-subunit in either phosphorylation state. | UniProt ID P11798 (mouse), P11275 (rat) | Unit of Measure (UM) µl