Ar space. We previously discovered that extracellular vesicles (EVs) from endothelial progenitor cells (EPCs) avert endothelial dysfunction and lung injury in sepsis as a consequence of their encapsulation of miRNA-126. Having said that, the effects of EPC EVs in acute lung injury (ALI) remains unknown. Procedures: To figure out if EPC EVs would have valuable effects in ALI, intratracheal administration of lipopolysaccharide (LPS) was employed to induce ALI in mice. Lung permeability, inflammation and the part of miRNA-126 in alveolar epithelial barrier function have been examined. Results: The intratracheal administration of EPC EVs lowered lung injury following LPS-induced ALI at 24 and 48 h. When compared with placebo, intratracheal administration of EPC EVs significantly reduced the cell quantity, protein concentration and cytokines/chemokines inside the bronchoalveolar lavage fluid, indicating a reduction in permeability and inflammation. Additional, EPC EVs decreased myeloperoxidase activity and lowered the lung injury score, demonstrating protection againstIntroduction: Trauma and degeneration of articular cartilage (AC) could trigger the morbidity of one of many top disabling illness, osteoarthritis (OA). One of several most complicated troubles in remedy will be the poor selfhealing potential of AC. Extracellular vesicle (EV) transplantation has received extra and much more attention as potential cell-free therapeutic approaches to market tissue healing. In our preliminary study, we located that decreased expression of hsa_circ_0000077 (circ77) was closely related to OA. And circ77-overexpression in chondrocytes can avoid the chondrocyte degeneration. In this study, EVs derived from circ77-overexpressing synovium mesenchymal stem cells (SMSC-77EVs) have been applied to promote cartilage regeneration. Strategies: CCK-8, qPCR and western blotting (WB) had been CD252/OX40 Ligand Proteins manufacturer utilised to investigate the biological functions of SMSC-77-EVs around the proliferation and cartilage regeneration. In addition, interleukin 1 (IL-1) were utilised to simulate the inflammatory situations of OA, and after that, the protective effects of SMSC-77-EVs have been confirmed by CCK-8, qPCR and WB. Final results: CCK-8 assay confirmed that SMSC-77-EVs enhanced the proliferation of chondrocytes, compared with regular control and EVs derived from synovium mesenchymal stem cells which were transfected by empty vectors (SMSC-Empty-EVs). WB and qPCR assays confirmed that SMSC-77-EVs enhanced theISEV2019 ABSTRACT BOOKexpression levels of cartilage related proteins including Kind II collagen (Col-II), aggrecan (ACAN) and SOX9, compared with standard handle and SMSC-Empty-EVs. IL-1 drastically inhibited the proliferation and cartilage regeneration-related proteins (Col-II, ACAN and SOX9). SMSC-77-EVs could observably restrain the dangerous effects of IL-1, when SMSC-Empty-EVs showed restricted ability. Summary/Conclusion: These findings suggest that the novel SMSC-77-EVs offers the preferable function in promoting the repair of cartilage harm. The usage of SMSC-77-EVs would represent a improvement trend of cell-free therapies, making use of engineered EVs (or modularized EVs), for promoting cartilage regeneration. Funding: The National Natural Science Foundation of China [Nos. 81871834, 81802226 and 81301589], and Shanghai Jiao Tong University K.C.Wong Healthcare Fellowship Fund supported this work.PT12.Lymphangiogenesis induced by exosomes derived from adiposederived mesenchymal stem cells Kensuke CD73 Proteins Gene ID Tashiroa, Yusuke Yoshiokab and Takahiro OchiyabaThe incubation time was 48 h in proliferation assa.