7.30; identified: C, 82.78, H, 7.31 . IDO2 Molecular Weight Methyl 2,three,4-tri-O-cinnamoyl-6-O-myristoyl–Dgalactopyranoside (8). FTIR (KBr) (max): 1702 (-CO) cm-1. 1H-NMR (CDCl3, 400 MHz) ( ppm): H 7.75 7.52, 7.37 (3 1H, three d, J = 16.0 Hz, 3 PhCH = CHCO-), 7.54 (6H, m, Ar ), 7.28 (9H, m, Ar ), six.55, 6.16, 6.07 (three 1H, three d, J = 16.1 Hz, 3 PhCH = CHCO-), five.48 (1H, d, J = eight.two Hz, H-1), five.34 (1H, dd, J = 8.2 and 10.6 Hz, H-2), five.05 (1H, dd, J = three.2 and 10.six Hz, H-3), 4.66 (1H, d, J = 3.7 Hz, H-4), 4.40 (1H, dd, J = 11.2 and six.6 Hz, H-6a), four.01 (1H, dd, J = 11.2 and six.eight Hz, H-6b), 3.52 (1H, m, H-5), 3.50 (3H, s, 1-OCH3), two.32 2H, m, CH 3(CH 2) 11CH 2CO-, 1.63 2H, m, CH3(CH2)10CH2CH2CO-, 1.25 20H, m, CH3(CH2)10CH2CH2CO-, 0.88 3H, m, CH3(CH2)12CO-. LC S [M + 1]+ 795.97. Anal Calcd. for C48H58O10: C, 72.52, H, 7.35; discovered: C, 72.53, H, 7.37 .Methyl 6-O-myristoyl-2,three,4-tri-O-(p-toluenesulfonyl)–Dgalactopyranoside (9). FTIR (KBr) (max): 1705 cm-1 (C = O). 1H-NMR (CDCl3, 400 MHz) ( ppm): H 8.03 (3 2H, m, Ar ), 7.94 (three 2H, m, Ar ), five.23 (1H, d, J = eight.2 Hz, H-1), five.08 (1H, dd, J = 8.0 and ten.5 Hz, H-2), four.77 (1H, dd, J = 3.1 and 10.6 Hz, H-3), four.53 (1H, d, J = 3.7 Hz, H-4), four.27 (1H, dd, J = 11.0 and six.five Hz, H-6a), four.11 (1H, dd, J = 11.1 and six.eight Hz, H-6b), 3.98 (1H, m, H-5), three.46 (3H, s, 1-OCH3), two.37 2H, m, CH3(CH2)11CH2CO-, 1.63 2H, m, CH3(CH2)10CH2CH2CO-, 1.27 20H, m, CH3(CH2)10CH2CH2CO-, 0.98 3H, m, CH3(CH2)12CO-. LC S [M + 1]+ 868.ten. Anal Calcd. for C42H58O13S3: C, 58.17, H, six.74; identified: C, 58.19, H, six.76 . Methyl 2,3,4-tri-O-(3-chlorobenzoyl)-6-O-myristoyl-D-galactopyranoside (ten). FTIR (KBr) (max): 1709 cm-1 (C = O). 1H-NMR (CDCl3, 400 MHz): H eight.05 (3H, m, Ar ), 7.96 (3H, m, Ar ), 7.55 (3H, m, Ar ), 7.38 (3H, m, Ar -H), five.63 (1H, d, J = eight.1 Hz, H-1), 5.21 (1H, dd, J = eight.2 and 10.six Hz, H-2), five.01 (1H, dd, J = 3.1 and 10.six Hz, H-3), 4.65 (1H, d, J = three.7 Hz, H-4), 4.40 (1H, dd, J = 11.1 and six.6 Hz, H-6a), 4.20 (1H, dd, J = 11.two and six.8 Hz, H-6b), four.00 (1H, m, H-5), three.46 (3H, s, 1-OCH3), 2.35 2H, m, CH3(CH2)11CH2CO-, 1.65 2H, m, CH3(CH2)10CH2CH2CO-, 1.24 20H, m, CH3(CH2)10CH2CH2CO-, 0.86 3H, m, CH3(CH2)12CO-. LC S [M + 1]+ 821.19. Anal Calcd. for C42H49O10Cl3: C, 61.50, H, 6.02; discovered: C, 61.52, H, six.03 .Antimicrobial screeningThe fifteen modified thymidine derivatives (20) were subjected to antibacterial screening utilizing 5 bacterial strains: two Gram-positive strains, namely, Bacillus subtilis ATCC 6633 and Staphylococcus aureus ATCC 6538, and three Gram-negative strains, namely, Escherichia coli ATCC 8739, Salmonella abony NCTC 6017 and Pseudomonas aeruginosa ATCC 9027. Each of the compounds were dissolved in dimethylformamide (DMSO) to get a two remedy (w/v). Moreover, antifungal activities in the compounds have been studied against two fungi strains, namely, Aspergillus niger ATCC 16,404 and Aspergillus flavus ATCC 204,304. These test micro-organisms (bacteria and fungi) have been obtained from the CCR2 web Division of Microbiology, University of Chittagong, Bangladesh. Disks soaked in DMSO were used as the unfavorable manage.Screening of antibacterial activityThe antibacterial spectra on the test derivatives were obtained in vitro by the disk diffusion method [29]. This technique applied paper disks of 4 mm diameter in addition to a glass Petri-plate of 90 mmGlycoconjugate Journal (2022) 39:261diameter throughout the experiment. Sterile five (w/v) dimethyl sulfoxide (DMSO) option prepared the synthesized compounds’ preferred concentration and regular antibiotics. The pa