Tion reaction among 4-(1,2,2-triphenylvinyl) vinyl)benzaldehyde and 2,2-thenil in the presence of ammonium acetate acetate (Scheme 1) and isolated benzaldehyde and two,two -thenil inside the presence of ammonium (Scheme 1) and isolated as a white strong in 85 in 85 yield. as a white solid yield.Scheme 1. Synthesis ofScheme 1. Synthesis from the molecular sensor DTITPE. the molecular sensor DTITPE.The 1H NMR spectrumNMR spectrum of DTITPE showedresonance at 9.30 due to at 9.30 due The 1 H of DTITPE Estramustine phosphate Biological Activity showed a broad singlet a broad singlet resonance the imidazole proton,imidazole proton, eight.two Hz) at 7.61, assignable at 7.61, assignable to two protons towards the in addition to a doublet (J = as well as a doublet (J = eight.two Hz) to two protons of the substituted aryl ring in the tetraphenylethylenethe tetraphenylethylenedue to theThe resonances resulting from in the substituted aryl ring of moiety. The resonances moiety. remaining aromatic and thienyl protons appear as multiplets about 7.0.5. The MALDI-TOF 7.0.5. The the remaining aromatic and thienyl protons seem as multiplets around mass spectrumMALDI-TOF mass spectrum of DTITPE showed the at m/z 563.six. of DTITPE showed the expected [M+H]+ ion peak expected [M+H]+ ion peak at m/z 563.six. The molecular The molecular structure of also confirmed by single-crystal X-ray dif- X-ray diffracstructure of DTITPE was DTITPE was also confirmed by single-crystal tion (Figure 2). Light-yellow colored square-shaped crystals of DTITPE, fraction (Figure two). Light-yellow colored square-shaped crystals of DTITPE, obtained from obtained from THF/hexane by slow evaporation, crystallized in Pna21 space group (TaTHF/hexane by slow evaporation, crystallized within the orthorhombic the orthorhombic Pna21 space group (Table confirmed the presence of TPE using a di(thienyl) substituted imidble S4). The structure S4). The structure confirmed the presence of TPE using a di(thienyl) substituted imidazole from the bound to a single of imidazole and attached phenyl ring are azole group bound to one group phenyl rings; the the phenyl rings; the imidazole and attached phenyl ring are almost co-planar. Inside the structure group containing S1 group of 15 nearly co-planar. Within the structure of DTITPE, the thienylof DTITPE, the thienylwas disorsensors 2021, 9, x FOR PEER Overview 6 containing S1 was disordered by a 180 rotation in regards to the C28 30 bond. angles in DTITPE and angles in the bond lengths dered by a 180rotation regarding the C28 30 bond. The bond lengths and DTITPE are are inside normal ranges. within typical ranges.Figure 2. Molecular structure of DTITPE. 50 probability levels. The thienyl group Figure 2. Molecular structure of DTITPE. Ellipsoids showEllipsoids show 50 probability levels. The thienyl group containing S1 (0.749:0.251) by a 180rotation a 180 rotation Lomeguatrib In Vitro concerning the C28 30 bond containing S1 was disorderedwas disordered (0.749:0.251) by about the C28 30 bond and only the and only the big position is shown. important position is shown.Upon the addition of TBAF to a DTITPE in THF, alterations alterations in the Upon the addition of TBAF to a remedy of resolution of DTITPE in THF,inside the NMR NMR spectrum had been observed. The 1 H NMR spectrumthe disappearance on the imid- the imidazole spectrum had been observed. The 1H NMR spectrum showed showed the disappearance of proton resonance and a downfield shift of o-phenyl proton proton resonances, from azole proton resonance and a downfield shift of the twothe two o-phenyl resonances, from 7.61 to eight.10 ppm, on account of a de-sh.