Centrifuged at 20,000 g for 90 min at 18 . The pellet of PMPs loaded with DOX (PMPDs) was resuspended in PAS. The sizes plus the concentrations of PMPs and PMPDs have been measured utilizing a nanoparticle tracking analysis (NTA). Data were analysed working with NTA software. Transportation of DOX from PMPDs to breast cancer cell lines was observed by deconvolution microscopy. Benefits: NTA final results revealed that the mean size of PMPDs (234.1 48.01 nm) was slightly larger compared with that of PMPs (200.1 57.71 nm) and that DOX incorporation didn’t influence the quantification of PMPs. The concentration of them was no significant difference. The size distributions and pictures of PMPs and PMPDs indicated the absence of aggregated PMPs linked with DOX loading. When incubated with MCF-7 and MDA-MB-231 cells, PMPDs transferred DOX to the nuclei of cancer cells within 30 min. Summary/Conclusion: These results help the possible clinical use of PMPDs as novel cell-based “Trojan Horse” anti-cancer therapeutic tactic. Funding: This study was supported by the Ministry of Science and Technologies.PT11.Style of an exosome-based drug delivery system transporting anticancer peptides for targeting breast metastases within the brain Filipa Oliveiraa, Julia Skalskaa, Tiago Figueiraa, Patr ia Napole a, ica Mellob, David Andreuc, Valdirene Gomesb, Miguel Castanhoa and Diana Gaspara Instituto de Medicina Molecular Jo Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, Lisboa, Portugal; bLaborat io de Fisiologia e Bioqu ica de Microrganismos do Centro de Bioci cias e Biotecnologia da Universidade Estadual do Norte Fluminense Darcy Ribeiro, Rio de Janeiro, Brazil; 3Department of Experimental and Health Sciences, Pompeu Fabra University, Barcelona Biomedical Research Park, Barcelona, Spainacharacterized with transmission electron microscopy (TEM), atomic force microscopy (AFM), flow cytometry, Western Blot and dynamic light scattering. The interaction of PvD1 and vCPP2319 ACPs with all the breast cells and respective exosomes was also followed with surface plasmon resonance (SPR) as to detail peptide’s binding to the distinct exosomes. Outcomes: Final results suggests an intracellular target for vCPP2319 cytotoxic activity on breast cancer cells. The binding on the peptides to both membranes of human cells and exosomes outcomes in cell death and in sturdy binding, respectively, pointing for the prospective potential of these breast exosomes in transporting ACPs, which in turn are very helpful towards tumour cells. Summary/Conclusion: Despite the fact that extra studies are currently in improvement, the mixture of possible ACPs with CD160 Proteins Synonyms human-derived exosomes are shown as a prospective supply for a hugely selective and successful DDS aiming to attack breast tumour cells positioned inside the brain. Funding: Funda o para a Ci cia e a Tecnologia (FCT I.P., Portugal) is acknowledged for funding PTDC/BBBBQB/1693/2014. F. O., J. S. and T. F. acknowledge FCT I.P., Portugal for fellowships PD/ BD/135046/2017, PD/BD/114177/2016 and SFRH/BD/ 5283/2013, respectively. Marie Sklodowska-Curie Analysis and Innovation Employees Exchange (RISE) is acknowledged for funding: call H2020-MCA-RISE2014, Grant agreement 644, 167, 2015019.PT11.Embryonic stem cells-derived exosomes endowed with targeting properties as chemotherapeutics delivery automobiles for glioblastoma therapy Xiaozheng Ling, Qingwei Zhu, Yunlong Yang, Yang Wang, BTN3A2 Proteins Recombinant Proteins Zhifeng Deng Shanghai Jiao Tong University Affliated Sixth People’s Hospital, Shanghai, Chin.