The specificity of Bax and Smac siRNA duplexes was evaluated by analyzing Bax and Smac protein expression in A2780s cells transfected with the corresponding shRNA construction (Determine 5C)
The quantitative assessment of sub-G1 cells by flow cytometry was applied to estimate the number of apoptotic cells. As proven in determine 3A, in cisplatin-delicate A2780s cells, the apoptotic cells…