Resulted peptides combination was submitted to robust cation exchange chromatography (SCX) adopted by titanium oxide (TiO2) affinity enrichment of phosphopeptides and then analyzed by LC-MS/MS
LAT regulates phosphorylation of ZAP70 and CD3Z. (A) The comparison of CD3f and ZAP70 phosphopeptides kinetics in CL20 and JCaM2.5 cell traces. (B) SILAC labeling approach for direct comparison of…