The proper sum of radiolabeled NF-kB oligonucleotide was added to every single sample and the sample was incubated at area temperature for extra twenty min
Soon after protein extraction from lung tissue, samples (15 mg plasmatic proteins) have been electrophoresed through SDS-polyacrylamide gel and transferred to polyvinylidene difluoride membranes (Millipore). WB assessment was carried out…