The stainless metal goal with 384 sample spots (with additional 13 calibration places) and a-cyano-four-hydroxycinnamic acid (five mg/ml) as MALDI matrix in 60% acetonitrile/.1% TFA (v/v) were used in all MALDI experiments
Sample collection and electrophoresis. 20 pairs of prepupal salivary glands ended up dissected from animals 8? hr APF and transferred to a new ten ml fall of Ringer (diluted 1:1)…