The DAPI-polyP curve (crimson) was rightward shifted in contrast to DAPI-DNA (green) and also matched carefully with the subtracted curve acquired from the vertebral growth plates (handle minus ALP, Determine 6B dashed line)
If the 520?80 nm DAPI-polyP signal in vertebral growth plates partly identifies polyPs, then application of intestinal ALP (to hydrolyze polyPs) need to change the emission profile. The DAPI emission…