Then the cells were harvested and plated once again on a 35 mm culture dish coated with laminin and cultured in a neuronal induction medium supplemented with Y-27632 for 21 times by changing the medium each and every three times
For differentiation of human iPSCs into neuronal cells, we employed the protocol as explained earlier and was even more modified in our lab. The hf-iPSCs ended up dissociated with Accutase…